Oligo synthesis: three key challenges for manufacturers
The market demands pure oligos – often within 24 hours. That leaves no scope for errors or failures. For customer-specific oligonucleotide synthesis, your synthesis device must dose up to 12 different liquids quickly and reliably. The key to success in this process:
- Reliable and reproducible dosing
The highly sensitive production process requires that errors be identified and rectified immediately. The quicker you can react, the more reliable are your processes.
- Ensuring the purity of oligos
The quality of an oligonucleotide depends on its full length and the correct order of nucleotides. Therefore, dosing must be performed quickly and with exceptional precision during oligonucleotide synthesis.
- Accelerating the manufacturing process
Time-pressure dosing enables you to increase the tempo and efficiency of the dosing process because it works more quickly than conventional solutions, such as syringe pumps. At the same time, you benefit from reproducible dosing and flexible dosing quantities.
We use these solutions to create added value for you
Using dosing solutions to boost the productivity of oligo manufacturing
Bürkert’s time-pressure dosing technology speeds up the dosing process and makes your synthesiser more efficient. Good to know: We tailor the modular systems to your requirements – or develop them together with you.
High-precision pressure controller
Exact pressure equalisation even at minimal flow rates
After the switching operation by the valve, the controller readjusts the pressure within milliseconds.
Deviation from set-point pressure or actual pressure
During the switching operation – illustrated by the bars in the diagram – the pressure inside the container drops. The curve shows how the controller readjusts the set-point pressure within milliseconds, by either applying pressure or venting pressure. This is a bidirectional closed-loop control procedure.
Our valve blocks: solutions for your dosing process
As an alternative to single valves, Bürkert block solutions offer many advantages, including fast integration and secure cabling. Furthermore, we integrate all the required features into the compact system for you.
Preventing defective oligos
The software with switching point detection serves as a permanent quality
control. It signals immediately if a valve has not switched. You can replace
it directly and avoid the wasteful production of defective oligos.
Thanks to the LEDs on the device, you can see directly where the error
has occurred and can rectify it immediately.
Guaranteeing reliable processes
Built-in pressure transmitters permanently monitor the pressure in the
valves and offer added reliability for your processes.
Real-time analysis or the digital communication interface allows you to
Saving time and materials
The block solution can be replaced quickly and requires far fewer cables
and plugs. For example, twelve valves can be supplied by just one electrical
connection. The components are permanently installed, thus preventing individual
wires from becoming loose.
From single valves to individually configured valve blocks – you will find the right solution for your dosing processes at Bürkert:
Real-time monitoring thanks to digital communication
With Bürkert valves, quality control is part of the dosing process: Thanks to the digital communication interface, you can monitor the production of oligos in real time. Signs of wear are detected immediately, and you can simply replace the valve.
Prevent defective oligos
The software with built-in switching point detection serves as a permanent quality control. It signals immediately if a valve has not switched. You can then simply replace the valve – and prevent defective oligos.
The easy way to find errors
The LEDs on the device allow you to see exactly where the error has occurred so you can rectify it immediately.
How Bürkert helps you detect pressure drops immediately
Bürkert solutions let you know at all times whether the pressure is correct. You can immediately see if the valve starts to leak due to wear. This is made possible by constant pressure monitoring and electrical switching point detection. The system reports the failure is immediately via the digital communication interface, allowing you to rectify it without delay.
Good to know: you only need to replace a valve when it is actually necessary.
Pressure curve for a process error
Pressure curve for a process error: The pressure is not fully built up again after switching. If this remains undetected, the oligonucleotides produced in the meantime will be defective.
Pressure curve of an intact valve
Pressure curve with no process error: The pressure immediately increases to the previous level after switching. The Bürkert valve solution indicates a pressure drop immediately.
How much time will failure detection save you during oligo production?
We have done the calculations for you.
The comparison shows how failure detection can get your production process up and running again quickly. Depending on your routines, you can save a lot of time between the occurrence of a pressure drop and its detection.
Conventional valve solution Bürkert system solution
A valve has not switched
(defective oligos will be produced from this point on)
The batch is checked and the error found
Half a day’s production is wasted
– and your scheduled delivery to the end customer is therefore in jeopardy
A valve has not switched – the operator recognises the error and eliminates it immediately.
The defective valve can be replaced immediately.
The customer receives high-purity oligos on schedule
Maximum quality and minimum time loss
(20 mins. compared to 4 hours without a Bürkert solution)
The comparison shows the minimal time loss as well as the maximum quality of the Bürkert system solution compared with the conventional solution. The schedules are met with the Bürkert solution.
Do you also want to produce high-quality DNA strands more quickly and efficiently? We look forward to hearing from you.
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Bürkert Systemhaus – where your ideas feel at home
Working in partnership with you, we develop individual solutions that precisely meet your expectations.
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